Yoon Hs, Golden JW (2001) PatS and Products of Nitrogen Fixation Control Heterocyst Pattern PatS and Products of Nitrogen Fixation Control Heterocyst Pattern. J Bacteriol 183: 2605–2613. pmid:11274121 Li JH (2003) An increase in the level of 2-oxoglutarate promotes heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 149: 3257–3263. pmid:14600238 Allard J, Hill A, Rutenberg A (2007) Heterocyst patterns without patterning proteins in cyanobacterial filaments. Dev Biol 312: 427–434. pmid:17976569 Sequencing reads from the experimental HetR-6xHis sample were randomly assigned to three files and the full ChIP-seq analysis was performed on each sample as a technical replicate. ChIP-seq reads from the three HetR-6xHis samples and the WT sample were aligned to NCBI's current build of the Anabaena genome with CLC Genomics Workbench 5. ChIP peaks were called using a 100 bp window and a false discovery rate of 5% with the WT sample as the control using CLC's ChIP Analysis pipeline. ChIP peaks were excluded from the final data set if they were not present in at least two of the three technical replicates. All peaks were verified by eye prior to further analysis. Promoter-gfp reporter fusion construction In this work, we develop a simple mathematical model by incorporating the recent experimental results on the genetic regulatory network of cyanobacteria into the theoretical machinery of system biology.

KUALA LUMPUR: Malaysia’s Hydrogen Economy and Technology Roadmap (HETR) has been launched, pushing the country to the forefront of energy transition and to becoming a regional leader in the renewable energy (RE) industry. Buchler NE, Gerland U, Hwa T (2003) On schemes of combinatorial transcription logic. Proc Natl Acad Sci 100: 5136–5141. pmid:12702751 Fig 5. States of a cyanobacterium when subjected to different conditions of nitrogen and diffusion.

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Meinhardt, H. Models of biological pattern formation: From elementary steps to the organization of embryonic axes. Curr. Top. Dev. Biol. 81, 1–63 (2008).

Süel GM, Garcia-Ojalvo J, Liberman LM, Elowitz MB (2006) An excitable gene regulatory circuit induces transient cellular differentiation. Nature 440: 545–550. pmid:16554821 Herrero, A., Picossi, S. & Flores, E. Gene expression during heterocyst differentiation. Adv. Bot. Res., 65, 281–329 (2013). In the previous section, we introduced a single cell model for the cyanobacteria reaction to nitrogen-limiting conditions. There we have shown that, for a specific range of parameters, the model exhibits features that would lead to heterocyst development under noisy conditions. Nevertheless, the model should be extended to cyanobacteria chains to account for heterocyst development since, as previously noted, isolated cyanobacteria do not become heterocysts by themselves; the action of the chain is needed to generate heterocysts. Ramasubramanian TS, Wei TF, Golden JW (1994) Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes. J Bacteriol 176: 1214–1223. pmid:8113160

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